Abstract
Toxoflavin (an azapteridine) is degraded to a single product by toxoflavin lyase (TflA) in a reaction dependent on reductant, Mn(II), and oxygen. The isolated product was fully characterized by NMR and MS and was identified as a triazine in which the pyrimidine ring was oxidatively degraded. A mechanism for toxoflavin degradation based on the identification of the enzymatic product and the recently determined crystal structure of toxoflavin lyase is proposed.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Lyases / chemistry
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Lyases / metabolism*
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Manganese / chemistry
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Manganese / metabolism
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Models, Molecular
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Molecular Structure
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Oxidation-Reduction
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Oxygen / chemistry
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Oxygen / metabolism
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Pyrimidinones / chemistry*
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Pyrimidinones / metabolism
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Triazines / chemistry*
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Triazines / metabolism
Substances
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Pyrimidinones
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Triazines
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Manganese
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toxoflavin
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Lyases
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Oxygen