Abstract
The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; a-D-GalpNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Neoplasm / immunology*
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Antigens, Tumor-Associated, Carbohydrate / immunology*
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Bacteroides fragilis / immunology*
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Breast Neoplasms / drug therapy
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Breast Neoplasms / immunology*
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Breast Neoplasms / pathology
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Cancer Vaccines / immunology*
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Carbohydrates / chemistry
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Carbohydrates / immunology
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Cell Line, Tumor
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Female
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Humans
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Immune Sera / immunology
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Immunity
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Immunization
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Immunoglobulin G / metabolism
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Interleukin-17 / metabolism
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Mice
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Mice, Inbred C57BL
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Polysaccharides, Bacterial / administration & dosage
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Polysaccharides, Bacterial / chemistry
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Polysaccharides, Bacterial / genetics
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Polysaccharides, Bacterial / immunology
Substances
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Antigens, Neoplasm
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Antigens, Tumor-Associated, Carbohydrate
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Cancer Vaccines
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Carbohydrates
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Immune Sera
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Immunoglobulin G
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Interleukin-17
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Polysaccharides, Bacterial
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Tn antigen