Estrogen effects on the forced swim test differ in two outbred rat strains

Physiol Behav. 2012 May 15;106(2):81-6. doi: 10.1016/j.physbeh.2012.01.004. Epub 2012 Jan 12.

Abstract

Changes in reproductive hormones, such as estrogen, play a role in mood regulation. The present study examined strain differences (Long-Evans vs. Wistar-Hannover) in the behavioral and biochemical effects of estrogen manipulation. Adult ovariectomized female rats were treated with estradiol, vehicle, or withdrawn from estradiol. The two strains demonstrated differential behavioral responses to short-term estradiol administration in the forced swim test; estradiol induced an antidepressant-like effect in Long-Evans rats but not in Wistar rats. Conversely, withdrawal from estradiol resulted in a depressive-like state in the Wistar rats but not in the Long-Evans rats. Western blot analyses found no differences in estrogen receptors α and β within the hippocampus or the frontal cortex, two brain areas strongly implicated in affective disorders. These data demonstrate the importance of strain as a variable when interpreting behavioral effects of estrogen.

MeSH terms

  • Animals
  • Estradiol / blood
  • Estradiol / pharmacology
  • Estrogen Receptor alpha / metabolism*
  • Estrogen Receptor beta / metabolism*
  • Estrogens / pharmacology
  • Estrogens / physiology*
  • Female
  • Frontal Lobe / drug effects
  • Frontal Lobe / metabolism
  • Hippocampus / drug effects
  • Hippocampus / metabolism
  • Immobility Response, Tonic / drug effects
  • Immobility Response, Tonic / physiology*
  • Rats
  • Rats, Long-Evans
  • Rats, Wistar
  • Species Specificity*
  • Substance Withdrawal Syndrome / psychology

Substances

  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Estrogens
  • Estradiol