Fruit consumption is encouraged for health reasons; however, fruits may harbour a series of allergenic proteins that may cause discomfort or even represent serious threats to certain individuals. Thus, the identification and characterization of allergens in fruits requires novel approaches involving genomic and proteomic tools. Since avoidance of fruits also negatively affects the quality of patients' lives, biotechnological interventions are ongoing to produce low allergenic fruits by down regulating specific genes. In this respect, the control of proteins associated with allergenicity could be achieved by fine tuning the spatial and temporal expression of the relevant genes.
Keywords: 2D-electrophoresis; Agrobacterium mediated transformation; ELISA, Immuno Tissue Printing; RNAi approach; RT-PCR; Southern Blot; Western Blot; pathogenesis-related proteins; small fruits; temperate fruits.