Using immunoenzymatic method (IE) for human immunodeficiency virus type 1 (HIV-1) serologic detection, and comparing with indirect immunofluorescence method (IIF). This method had same specificity and reproducibility but more sensitivity than IIF. Because it is simple, practical and suitable, IE method can replace IIF as the HIV serologic screening method suited to base application. Using HIV-1 immunoenzymatic reagent kit in Ivory Coast obtained good results.