Development of a fast ELISA for quantifying polio D-antigen in in-process samples

R ten Have; Y E Thomassen; M R J Hamzink; W A M Bakker; O E M Nijst; G Kersten; G Zomer

doi:10.1016/j.biologicals.2011.11.004

Development of a fast ELISA for quantifying polio D-antigen in in-process samples

Biologicals. 2012 Jan;40(1):84-7. doi: 10.1016/j.biologicals.2011.11.004. Epub 2011 Dec 6.

Authors

R ten Have¹, Y E Thomassen, M R J Hamzink, W A M Bakker, O E M Nijst, G Kersten, G Zomer

Affiliation

¹ Unit Vaccinology, CIb, Centre for Infectious Disease Control, RIVM, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Rimko.ten.Have@RIVM.nl

PMID: 22154015
DOI: 10.1016/j.biologicals.2011.11.004

Abstract

A fast ELISA was developed and qualified for analysis of polio D-antigen. The original 20 h-protocol was optimized by minimizing the total incubation time to 1 h, and by replacing the signal reagent 3,3',5,5'-tetramethylbenzidine by a chemiluminogenic signal reagent with a theoretical low intrinsic background and high dynamic range.

MeSH terms

Antigens, Viral / analysis
Antigens, Viral / immunology*
Enzyme-Linked Immunosorbent Assay / methods
Enzyme-Linked Immunosorbent Assay / standards
Luminescent Measurements / methods*
Luminescent Measurements / standards
Poliovirus / immunology*

Substances

Antigens, Viral