Abstract
The bromodomain protein BRD4 is involved in cell proliferation and cell cycle progression, primarily through its role in acetylated chromatin-dependent regulation of transcription at targeted loci. Here, we show that BRD4 is frequently downregulated by aberrant promoter hypermethylation in human colon cancer cell lines and primary tumors. Ectopic re-expression of BRD4 in these colon cancer cell lines markedly reduced in vivo tumor growth, suggesting a role of BRD4 in human colon cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Animals
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Cell Cycle Proteins
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Cell Line, Tumor
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Cell Transformation, Neoplastic / genetics
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Cell Transformation, Neoplastic / pathology
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Colonic Neoplasms / genetics*
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DNA Methylation / genetics
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Epigenesis, Genetic*
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Gene Expression Regulation, Neoplastic*
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Gene Silencing
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Histones / metabolism
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Humans
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Mice
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic / genetics
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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BRD4 protein, human
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Cell Cycle Proteins
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Histones
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Nuclear Proteins
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Repressor Proteins
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Transcription Factors