Diabetic nephropathy-related active cyclic peptides from the roots of Brachystemma calycinum

Bioorg Med Chem Lett. 2011 Dec 15;21(24):7434-9. doi: 10.1016/j.bmcl.2011.10.004. Epub 2011 Oct 12.

Abstract

Three new cyclic peptides, namely duanbanhuains A-C (1-3), were isolated from the roots of Brachystemma calycinum which is a traditional medicine used to treat rheumatic diseases. Their structures were identified by means of a suite of MS and NMR experiments. These compounds were purposely evaluated for their inhibitory effects on the release of MCP-1, IL-6, collagen IV and reactive oxygen species (ROS) against high-glucose-stimulated mesangial cells. The results showed that compounds 1 and 2 exhibited potent inhibition on the production of IL-6, collagen IV and ROS at the concentration of 10 μM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Caryophyllaceae / chemistry*
  • Cells, Cultured
  • Collagen Type IV / metabolism
  • Diabetic Nephropathies
  • Glucose / pharmacology
  • Humans
  • Interleukin-6 / metabolism
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Mesangial Cells / cytology
  • Mesangial Cells / drug effects
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / isolation & purification
  • Peptides, Cyclic / pharmacology
  • Plant Roots / chemistry
  • Protein Conformation
  • Reactive Oxygen Species / metabolism
  • Receptors, CCR2 / metabolism

Substances

  • Collagen Type IV
  • Interleukin-6
  • Peptides, Cyclic
  • Reactive Oxygen Species
  • Receptors, CCR2
  • duanbanhuain A
  • duanbanhuain B
  • Glucose