Microcalorimetric study of the growth of bacterial colonies of Lactococcus lactis IL1403 in agar gels

Abstract

Growth of Lactococcus lactis IL1403 in solid agar gels and liquid cultures at different glucose concentrations of 2, 10 and 20 g/L and different inoculation rates from 10(0) to 10(6) cfu/mL with the 10-fold increment was studied using thermal activity monitor TAM III. In parallel to calorimetric measurements the changes of glucose and lactic acid concentrations and pH of culture media were measured in order to obtain additional information for the interpretation of calorimetric power-time curves. Maximal specific growth rates of heat evolution proportional to growth rates of biomass μ(max) (W/h), heat produced during different growth stages Q(TOT) (J/mL), Q(ExP) (J/mL) and duration of lag-phases λ (h) were obtained by processing calorimetric curves. The sizes of colonies were measured also at the end of growth using microscope. The data obtained together with calculated heat yield coefficient Y(Q) (J/cfu) allowed to analyze and describe quantitatively the growth of individual colonies and develop a model of multistage growth of a typical colony of L. lactis in 1% agar gel. Microcalorimetry used in combination with other relevant methods is a very powerful and precise tool in studying solid-state fermentations.