Vanadium bromoperoxidase from Delisea pulchra: enzyme-catalyzed formation of bromofuranone and attendant disruption of quorum sensing

Chem Commun (Camb). 2011 Nov 28;47(44):12086-8. doi: 10.1039/c1cc15605e. Epub 2011 Oct 17.

Abstract

Vanadium bromoperoxidase was isolated and cloned from the marine red alga Delisea pulchra. This enzyme catalyzes the bromolactonization of 4-pentynoic acid forming 5E-bromo-methylidenetetrahydro-2-furanone, a compound which is shown herein to inhibit quorum sensing in the engineered reporter strain, Agrobacterium tumefaciens NTL4.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Agrobacterium tumefaciens / drug effects
  • Agrobacterium tumefaciens / physiology
  • Furans / chemistry
  • Furans / pharmacology*
  • Peroxidases / chemistry*
  • Peroxidases / isolation & purification
  • Peroxidases / physiology
  • Quorum Sensing / drug effects*
  • Rhodophyta / enzymology*
  • beta-Galactosidase / metabolism

Substances

  • Furans
  • Peroxidases
  • bromide peroxidase
  • beta-Galactosidase