Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method

Liang Cui; Xiaoyan Lin; Ninghang Lin; Yanling Song; Zhi Zhu; Xi Chen; Chaoyong James Yang

doi:10.1039/c1cc15412e

Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method

Chem Commun (Camb). 2012 Jan 7;48(2):194-6. doi: 10.1039/c1cc15412e. Epub 2011 Oct 5.

Authors

Liang Cui¹, Xiaoyan Lin, Ninghang Lin, Yanling Song, Zhi Zhu, Xi Chen, Chaoyong James Yang

Affiliation

¹ The Key Laboratory of Analytical Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

PMID: 21971052
DOI: 10.1039/c1cc15412e

Abstract

Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples. By using the quenching nature of graphene oxide for multiple fluorophores, this method can distinguish highly similar miRNA sequences and detect them simultaneously.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
DNA Probes / chemistry*
Deoxyribonuclease I / metabolism
Fluorescent Dyes / chemistry*
Graphite / chemistry*
Humans
MicroRNAs / analysis*
MicroRNAs / metabolism
Oxides / chemistry*
Sensitivity and Specificity

Substances

DNA Probes
Fluorescent Dyes
MicroRNAs
Oxides
Graphite
Deoxyribonuclease I