[Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application]

Nan Fang Yi Ke Da Xue Xue Bao. 2011 Sep;31(9):1509-12.
[Article in Chinese]

Abstract

Objective: To develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.

Methods: A double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.

Results: The sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.

Conclusion: The TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross Reactions
  • Fluoroimmunoassay / methods*
  • Pertussis Toxin / analysis*
  • Pertussis Vaccine / chemistry
  • Pertussis Vaccine / standards
  • Quality Control
  • Sensitivity and Specificity

Substances

  • Pertussis Vaccine
  • pertussis toxin, S1 subunit
  • Pertussis Toxin