Glycosylphosphatidylinositols of Toxoplasma gondii induce matrix metalloproteinase-9 production and degradation of galectin-3

Immunobiology. 2012 Jan;217(1):61-4. doi: 10.1016/j.imbio.2011.08.001. Epub 2011 Aug 12.

Abstract

Toxoplasma gondii glycosylphosphatidylinositols (GPIs) bind to galectin-3 to induce TNF-α production in macrophages via Toll-like receptors 2 and 4. Here we show that T. gondii GPIs stimulate human macrophages to synthesize matrix metalloproteinase-9 in a TNF-α-dependent pathway and degrade extracellular galectin-3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Carbohydrate Conformation
  • Cells, Cultured
  • Chlorocebus aethiops
  • Galectin 3 / immunology
  • Galectin 3 / metabolism*
  • Glycosylphosphatidylinositols / immunology
  • Glycosylphosphatidylinositols / metabolism*
  • Humans
  • Macrophage Activation / immunology*
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Matrix Metalloproteinase 9 / biosynthesis
  • Matrix Metalloproteinase 9 / immunology
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Binding
  • Signal Transduction / immunology*
  • Toll-Like Receptor 2 / immunology
  • Toll-Like Receptor 2 / metabolism
  • Toll-Like Receptor 4 / immunology
  • Toll-Like Receptor 4 / metabolism
  • Toxoplasma / chemistry
  • Toxoplasma / immunology
  • Toxoplasma / metabolism*
  • Toxoplasmosis / immunology
  • Toxoplasmosis / metabolism*
  • Toxoplasmosis / pathology
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism
  • Vero Cells

Substances

  • Galectin 3
  • Glycosylphosphatidylinositols
  • TLR2 protein, human
  • TLR4 protein, human
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Tumor Necrosis Factor-alpha
  • Matrix Metalloproteinase 9