Multilocus variable number tandem repeat analysis (MLVA) of Streptococcus pyogenes

Katarzyna Obszańska; Anna L Borek; Radosław Izdebski; Waleria Hryniewicz; Izabela Sitkiewicz

doi:10.1016/j.mimet.2011.08.017

Multilocus variable number tandem repeat analysis (MLVA) of Streptococcus pyogenes

J Microbiol Methods. 2011 Nov;87(2):143-9. doi: 10.1016/j.mimet.2011.08.017. Epub 2011 Sep 3.

Authors

Katarzyna Obszańska¹, Anna L Borek, Radosław Izdebski, Waleria Hryniewicz, Izabela Sitkiewicz

Affiliation

¹ Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Chełmska 30/34, 00-725 Warszawa, Poland.

PMID: 21920391
DOI: 10.1016/j.mimet.2011.08.017

Abstract

We tested 21 polymorphic loci encoded by the genome of Streptococcus pyogenes (group A Streptococcus, GAS). Seven of them were chosen for the MLVA scheme. The primer pairs, designed for selected loci, detect from few to several alleles, and the method has a Simpson's Index of diversity of 0.957. To test the overall performance of the method, multiplex PCR reactions were carried out for over 700 GAS strains. Using the method we were able to detect differences between highly clonal strains that share the same emm, MLST and PFGE types. The most diverse strains were M4, M2, M3 and M28. We developed a typing method that can be employed to differentiate between GAS strains. The method has high resolution and measures diversity of the GAS core chromosome, on the contrary to methods such as PFGE.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods*
Humans
Minisatellite Repeats*
Polymerase Chain Reaction / methods*
Streptococcal Infections / microbiology
Streptococcus pyogenes / classification
Streptococcus pyogenes / genetics
Streptococcus pyogenes / isolation & purification*