Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures

Cédric Sarazin; Didier Thiébaut; Patrick Sassiat; Jérôme Vial

doi:10.1002/jssc.201100332

Feasibility of ultra high performance supercritical neat carbon dioxide chromatography at conventional pressures

J Sep Sci. 2011 Oct;34(19):2773-8. doi: 10.1002/jssc.201100332. Epub 2011 Aug 24.

Authors

Cédric Sarazin¹, Didier Thiébaut, Patrick Sassiat, Jérôme Vial

Affiliation

¹ CNRS UMR 7195 PECSA, Laboratoire Sciences Analytiques, Bioanalytiques et Miniaturisation, ESPCI ParisTech, Paris, France.

PMID: 21898804
DOI: 10.1002/jssc.201100332

Abstract

The implementation of columns packed with sub-2 μm particles in supercritical fluid chromatography (SFC) is described using neat carbon dioxide as the mobile phase. A conventional supercritical fluid chromatograph was slightly modified to reduce extra column band broadening. Performances of a column packed with 1.8 μm C18-bonded silica particles in SFC using neat carbon dioxide as the mobile phase were compared with results obtained in ultra high performance liquid chromatography (UHPLC) using a dedicated chromatograph. As expected and usual in SFC, higher linear velocities than in UHPLC must be applied in order to reach optimal efficiency owing to higher diffusion coefficient of solutes in the mobile phase; similar numbers of theoretical plates were obtained with both techniques. Very fast separations of hydrocarbons are presented using two different alkyl-bonded silica columns.