Abstract
We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Cadmium Compounds / chemistry
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Carbocyanines / chemistry
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DNA / analysis*
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Fluorescence Resonance Energy Transfer*
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Nucleic Acid Hybridization
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotide Probes / chemistry*
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Quantum Dots*
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Selenium Compounds / chemistry
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Sulfides / chemistry
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Zinc Compounds / chemistry
Substances
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Cadmium Compounds
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Carbocyanines
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Oligonucleotide Probes
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Selenium Compounds
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Sulfides
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Zinc Compounds
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cyanine dye 5
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DNA
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cadmium selenide
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zinc sulfide