We previously showed that thymocytes express high levels of c-ets-1 protein (Ets1) that can be rapidly phosphorylated following mitogenic stimulation using lectins. We demonstrate here that T cell receptor (TCR) specific stimulation with monoclonal antibodies of mature CD8+ or CD4+ T cells also results in the rapid phosphorylation of Ets1, reinforcing the hypothesis of a possible role for Ets1 in T cell activation. In addition to the major Ets1 product (mu-p63c-ets-1), we identify in mouse thymocytes and mature T cells a distinct 52 Kd Ets1 related protein (mu-p52c-ets-1). In contrast to the major Ets1 protein, mu-p52c-ets-1 is poorly phosphorylated in unstimulated cells. Furthermore, mitogenic stimulation of thymocytes and T cells failed to induce in mu-p52c-ets-1 the Ca2(+)-dependent phosphorylation events which are known to drastically affect the migration of the major Ets1 protein in SDS polyacrylamide gels. Mu-p52c-ets-1, like mu-p63c-ets-1, is a nuclear-chromatin associated protein which exhibits DNA binding activity in vitro. However, in contrast to the major Ets1 protein, the association of mu-p53c-ets-1 with chromatin and its ability to bind to DNA in vitro are unaffected by activation stimuli resulting in an increase in [Ca2+]i. Finally, we present indications suggesting that mu-p52c-ets-1 might be the murine equivalent of the translation product of an alternatively c-ets-1 spliced mRNA described in human cells by others.