Abstract
Budding yeast is often used in chemical genetics for screening, target identification, and compound verification, but its high-level drug resistance has made the analysis of compounds difficult. Here we report the construction of 12geneΔ0HSR, a strain that lacks eight efflux pumps located on the plasma membrane and four transcription factors involved in expression of efflux pumps, and contains the RME1(ins-308A) mutation. This strain retained sufficient transformation, mating, and sporulation efficiency for genetic analysis in addition to hypersensitivity against several compounds. 12geneΔ0HSR is a useful tool for chemical biology, not only in chemical screening but in target identification and verification of bioactive compounds.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antifungal Agents / pharmacology*
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Gene Deletion*
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Gene Expression Regulation, Fungal
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Genetic Engineering / methods*
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Inhibitory Concentration 50
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Microbial Sensitivity Tests*
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Multidrug Resistance-Associated Proteins / deficiency*
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Multidrug Resistance-Associated Proteins / genetics
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Mutation
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Organisms, Genetically Modified / genetics
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Organisms, Genetically Modified / metabolism*
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Plasmids
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Transcription Factors / deficiency*
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Transcription Factors / genetics
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Transduction, Genetic
Substances
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Antifungal Agents
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Multidrug Resistance-Associated Proteins
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Transcription Factors