Comparison of real-time reverse-transcription loop-mediated isothermal amplification and real-time reverse-transcription polymerase chain reaction for detection of noroviruses in municipal wastewater

Yoshihiro Suzuki; Shogo Narimatsu; Takashi Furukawa; Akira Iwakiri; Miho Miura; Shogo Yamamoto; Hiroyuki Katayama

doi:10.1016/j.jbiosc.2011.06.012

Comparison of real-time reverse-transcription loop-mediated isothermal amplification and real-time reverse-transcription polymerase chain reaction for detection of noroviruses in municipal wastewater

J Biosci Bioeng. 2011 Oct;112(4):369-72. doi: 10.1016/j.jbiosc.2011.06.012. Epub 2011 Aug 6.

Authors

Yoshihiro Suzuki¹, Shogo Narimatsu, Takashi Furukawa, Akira Iwakiri, Miho Miura, Shogo Yamamoto, Hiroyuki Katayama

Affiliation

¹ Department of Civil and Environmental Engineering, University of Miyazaki, Miyazaki 889-2192, Japan. suzuki@civil.miyazaki-u.ac.jp

PMID: 21821471
DOI: 10.1016/j.jbiosc.2011.06.012

Abstract

The monitoring of NVs in municipal wastewater by both real-time RT-LAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1<p) for both NV-GI and NV-GII.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Environmental Monitoring / methods*
Norovirus / genetics
Norovirus / isolation & purification*
RNA, Viral / analysis
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Waste Disposal, Fluid
Water Microbiology*

Substances

RNA, Viral