Preparation and cell response of bio-mineralized Fe3O4 nanoparticles

J Colloid Interface Sci. 2011 Nov 1;363(1):393-402. doi: 10.1016/j.jcis.2011.07.009. Epub 2011 Jul 23.

Abstract

Silk fibroin (SF)-coated Fe(3)O(4) nanoparticles (NPs) with good superparamagnetism were successfully prepared via a bio-mineralization process at room temperature. Two cell tests revealed that mineralized SF-coated Fe(3)O(4) NPs presented good cytocompatibility for L929 and osteoblast cells and higher cell density after 5 d with high concentrations of SF-coated Fe(3)O(4) NPs (up to 0.5 mg/mL). These resulted from SF surface coating on NPs, nano-surface morphology and iron ion release of Fe(3)O(4) NPs. The mineralized SF-coated Fe(3)O(4) NPs could be envisioned for various bone orthopedic and therapeutic applications, in which SF-coated NPs location is controlled through an external magnetic field to promoted bone growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx / chemistry
  • Cell Line
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroins / chemistry
  • Magnetite Nanoparticles / chemistry*
  • Mice
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Particle Size
  • Silk / chemistry
  • Structure-Activity Relationship
  • Surface Properties

Substances

  • Magnetite Nanoparticles
  • Silk
  • Fibroins