Self-Assembly Pluronic and β-Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery

Min-Min Fan; Xi Zhang; Jie Qin; Bang-Jing Li; Xun Sun; Sheng Zhang

doi:10.1002/marc.201100272

Self-Assembly Pluronic and β-Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery

Macromol Rapid Commun. 2011 Oct 4;32(19):1533-8. doi: 10.1002/marc.201100272. Epub 2011 Jul 22.

Authors

Min-Min Fan¹, Xi Zhang¹, Jie Qin¹, Bang-Jing Li², Xun Sun³, Sheng Zhang⁴

Affiliations

¹ State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
² Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China. libj@cib.ac.cn.
³ West China School of Pharmacy, Sichuan University, Chengdu 610041, China. xunsun22@gmail.com.
⁴ State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China. zslbj@163.com.

PMID: 21786359
DOI: 10.1002/marc.201100272

Abstract

This paper studies a kind of hollow nanospheres prepared by self-assembly β-cyclodextrins (β-CDs) and poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (pluronic F127) for gene delivery. It was found that this kind of hollow nanospheres enable load PEI10K/DNA and the resulting F127NH2 βCD/(PEI10K/DNA) with 0.08 µg/well DNA display equal or higher gene delivery capability compared to PEI10K/DNA with 1 µg/well DNA in the absence or presence of serum. The cytotoxicity of the nanospheres was over 100 times lower than that of PEI10K.

Keywords: cyclodextrins; dendrimer; gene delivery; self-assembly; supramolecular hollow nanospheres.

MeSH terms

Gene Transfer Techniques*
Hep G2 Cells
Humans
Nanospheres / chemistry*
Poloxamer / chemistry*
beta-Cyclodextrins / chemistry*

Substances

beta-Cyclodextrins
Poloxamer