The piglet is an important animal model in biomedical research; many aspects of its anatomy, physiology and metabolism are similar to those of the human neonate. The authors describe a neonatal intensive care unit (NICU) for piglets. This unit allows researchers to model neonatal care in the NICU and can be used for a range of research studies. The authors hope that the model they describe can serve as a template for other investigators who would like to design their own piglet NICUs.