Limitations of spoligotyping and variable-number tandem-repeat typing for molecular tracing of Mycobacterium bovis in a high-diversity setting

J Clin Microbiol. 2011 Sep;49(9):3361-4. doi: 10.1128/JCM.00301-11. Epub 2011 Jul 13.

Abstract

This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Camelids, New World
  • DNA, Bacterial / genetics
  • Disease Outbreaks*
  • Genetic Variation*
  • Minisatellite Repeats
  • Molecular Epidemiology
  • Molecular Typing*
  • Mycobacterium bovis / classification*
  • Mycobacterium bovis / genetics*
  • Mycobacterium bovis / isolation & purification
  • Spain / epidemiology
  • Tuberculosis / epidemiology
  • Tuberculosis / microbiology
  • Tuberculosis / veterinary*

Substances

  • DNA, Bacterial