Abstract
Vaccinia virus recombinants co-expressing the genes for IL-2 and HSV-1 gC or gD were used to study the potential adjuvant effects of IL-2 on immunity to HSV-1. In addition, constructs were produced which, while expressing IL-2, encoded for reduced levels of HSV-1 gC. Studies with mice revealed that gC and gD could stimulate HSV-1 immunity as measured by neutralizing antibody (NA), lymphoproliferation and viral clearance. IL-2 enhancement of NA and lymphoproliferation was observed only in those groups of mice immunized with the sub-optimal gC construct which co-expressed IL-2. In no instance did the presence of IL-2 augment the ability of mice to clear an epithelial challenge of HSV-1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Electrophoresis, Polyacrylamide Gel
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Fluorescent Antibody Technique
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Gene Expression
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Herpes Simplex / immunology
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Herpes Simplex / prevention & control
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Immunity
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Immunization
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In Vitro Techniques
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Interleukin-2 / biosynthesis
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Interleukin-2 / genetics
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Interleukin-2 / immunology*
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Lymphocyte Activation / immunology
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Mice
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Neutralization Tests
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / immunology
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Simplexvirus / genetics
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Simplexvirus / growth & development
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Simplexvirus / immunology*
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
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Vaccinia virus / genetics
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Vaccinia virus / immunology*
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Vero Cells
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Viral Envelope Proteins / biosynthesis
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
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Viral Proteins / biosynthesis
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Viral Proteins / genetics
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Viral Vaccines / genetics
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Viral Vaccines / immunology*
Substances
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Interleukin-2
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Proteins
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Viral Vaccines
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glycoprotein gC, herpes simplex virus type 1