Ultrasonic nebulization extraction coupled with headspace single-drop microextraction of volatile and semivolatile compounds from the seed of Cuminum cyminum L

Abstract

Ultrasonic nebulization extraction (UNE) coupled with headspace single-drop microextraction (HS-SDME) was developed. In the UNE process, the analytes were transferred from the aqueous phase to the gas phase. Then the analytes were transferred from the gas phase to the solvent phase by the carrier gas and extracted and enriched with suspended microdrop solvent. Finally, the microdrop solvent injected into GC-MS system. The parameters affecting extraction performance, such as type of suspended solvent, microdrop volume, flow rate of carrier gas, temperature of extraction vessel and extraction time were investigated and optimized. The proposed method can be applied for the extraction and enrichment of the volatile and semivolatile compounds simultaneously. The extraction efficiency of the proposed method was compared with that of ultrasonic extraction (UE) and UE-HS-SDME. Compared with UE-HS-SDME, the contents of constituents in the extract obtained by the proposed method were closer to those obtained by hydrodistillation (HD), which is a standard extraction method.