12-O-tetradecanoylphorbol 13-acetate potentiates the action of cAMP in inducing DNA cleavage in thymocytes

K Suzuki; H Kizaki; T Tadakuma; Y Ishimura

doi:10.1016/0006-291x(90)91220-m

12-O-tetradecanoylphorbol 13-acetate potentiates the action of cAMP in inducing DNA cleavage in thymocytes

Biochem Biophys Res Commun. 1990 Sep 14;171(2):827-31. doi: 10.1016/0006-291x(90)91220-m.

Authors

K Suzuki¹, H Kizaki, T Tadakuma, Y Ishimura

Affiliation

¹ Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.

PMID: 2169735
DOI: 10.1016/0006-291x(90)91220-m

Abstract

12-O-Tetradecanoylphorbol 13-acetate (TPA) potentiated the action of cAMP in DNA cleavage in thymocytes induced by a low concentration of adenosine receptor-site agonists such as adenosine, 2-chloroadenosine and forskolin. The enhancement of DNA cleavage by TPA was also observed in dibutyryl cAMP-treated thymocytes. On the other hand, TPA suppressed accumulation of cAMP by the adenosine receptor-site agonists. These results suggest that activation of protein kinase C inhibits cAMP production, but stimulates cAMP-triggered process to induce DNA cleavage and death of thymocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

2-Chloroadenosine / pharmacology
Adenosine / pharmacology
Animals
Bucladesine / pharmacology
Calcium / pharmacology
Cells, Cultured
Colforsin / pharmacology
Cyclic AMP / physiology*
DNA / drug effects
DNA / metabolism*
Kinetics
Male
Mice
Mice, Inbred BALB C
T-Lymphocytes / drug effects
T-Lymphocytes / metabolism*
Tetradecanoylphorbol Acetate / pharmacology*

Substances

2-Chloroadenosine
Colforsin
Bucladesine
DNA
Cyclic AMP
Adenosine
Tetradecanoylphorbol Acetate
Calcium