Synthesis of a difunctional orthogonal coupler for the preparation of carbohydrate-functionalized sP(EO-stat-PO) hydrogels

Macromol Biosci. 2011 Sep 9;11(9):1201-10. doi: 10.1002/mabi.201100041. Epub 2011 Jun 16.

Abstract

The synthesis and characterization of a new difunctional coupler (4) based on trimethylolpropane (TMP) are described. The coupler is used to connect biologically active N-acetylglucosamine (GlcNAc) on amino-reactive microtiter plates and on star-shaped poly[(ethylene oxide)-stat-(propylene oxide)] hydrogel layers in microtiter plates. The biological activity of immobilized carbohydrates is determined using an enzyme-linked lectin assay.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosamine / chemistry
  • Carbohydrates / chemistry*
  • Cross-Linking Reagents / chemistry*
  • Hydrogels / chemical synthesis*
  • Hydrogels / chemistry
  • Lectins / analysis
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Polyethylene Glycols / chemistry*

Substances

  • Carbohydrates
  • Cross-Linking Reagents
  • Hydrogels
  • Lectins
  • Polyethylene Glycols
  • Acetylglucosamine