Abstract
Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Cryptococcus gattii / chemistry*
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Cryptococcus gattii / classification*
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Cryptococcus neoformans / chemistry*
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Cryptococcus neoformans / classification*
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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Humans
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Microbiological Techniques / methods*
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Mycology / methods*
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Sensitivity and Specificity
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Sequence Analysis, DNA / methods
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*