DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA

Xinjian Yang; Fang Pu; Jinsong Ren; Xiaogang Qu

doi:10.1039/c1cc12216a

DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA

Chem Commun (Camb). 2011 Jul 28;47(28):8133-5. doi: 10.1039/c1cc12216a. Epub 2011 May 31.

Authors

Xinjian Yang¹, Fang Pu, Jinsong Ren, Xiaogang Qu

Affiliation

¹ State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

PMID: 21629944
DOI: 10.1039/c1cc12216a

Abstract

We have demonstrated a DNA-templated small molecule ensemble that allows the label-free fluorescence turn-on detection of enzymatic or oxidative cleavage of single-stranded DNA with high sensitivity and selectivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Damage
DNA, Single-Stranded / genetics
DNA, Single-Stranded / metabolism*
Oxidation-Reduction
Single-Strand Specific DNA and RNA Endonucleases / metabolism*
Spectrometry, Fluorescence / methods*
Time Factors

Substances

DNA, Single-Stranded
Single-Strand Specific DNA and RNA Endonucleases