Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance

Lenka Dubska; Katerina Pilatova; Monika Dolejska; Zbynek Bortlicek; Tereza Frostova; Ivan Literak; Dalibor Valik

doi:10.1016/j.anaerobe.2011.05.008

Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance

Anaerobe. 2011 Dec;17(6):444-7. doi: 10.1016/j.anaerobe.2011.05.008. Epub 2011 May 23.

Authors

Lenka Dubska¹, Katerina Pilatova, Monika Dolejska, Zbynek Bortlicek, Tereza Frostova, Ivan Literak, Dalibor Valik

Affiliation

¹ Department of Laboratory Medicine, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic. dubska@mou.cz

PMID: 21624485
DOI: 10.1016/j.anaerobe.2011.05.008

Abstract

Based on experiments with 10 defined strains of Escherichia coli, we present a new method for bacterial phenotyping using SELDI-TOF mass spectrometry. Changes in bacterial protein profiles in the context of the time of cultivation and the antibiotic environment were minimal. Proteom subprofiling may further distinguish between strains with specific susceptibility to antimicrobials. Mass spec-based methods may become common in the future of bacterial pathogen identification in clinical microbiology diagnostics.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Drug Resistance, Bacterial*
Escherichia coli / chemistry*
Escherichia coli Proteins / analysis*
Humans
Microbial Sensitivity Tests / methods
Proteome / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Escherichia coli Proteins
Proteome