A recombinant vaccinia virus expressing the major capsid protein of Simian rotavirus-induced anti-rotavirus antibodies

Virus Res. 1990 Mar;15(3):267-74. doi: 10.1016/0168-1702(90)90034-9.

Abstract

cDNA molecules encoding the major structural protein (VP6) of the Simian rotavirus SA11 were inserted under the control of the vaccinia virus 7.5 kDa promoter into the thymidine kinase gene. Synthesis of VP6 was demonstrated by immunoprecipitation of recombinant virus-infected cell. Mice inoculated via several routes with this recombinant vaccinia produce high titers of antirotavirus antibodies lacking neutralizing activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / biosynthesis*
  • Antigens, Viral*
  • Capsid / genetics
  • Capsid / immunology*
  • Capsid Proteins*
  • DNA / genetics
  • Female
  • Genetic Vectors
  • Mice
  • Mice, Inbred BALB C
  • Recombination, Genetic
  • Rotavirus / genetics
  • Rotavirus / immunology*
  • Vaccination
  • Vaccinia virus / genetics*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • VP6 protein, Rotavirus
  • DNA