Dehydrogenase GRD1 represents a novel component of the cellulase regulon in Trichoderma reesei (Hypocrea jecorina)

Appl Environ Microbiol. 2011 Jul;77(13):4553-63. doi: 10.1128/AEM.00513-11. Epub 2011 May 20.

Abstract

Trichoderma reesei (Hypocrea jecorina) is nowadays the most important industrial producer of cellulase and hemicellulase enzymes, which are used for pretreatment of cellulosic biomass for biofuel production. In this study, we introduce a novel component, GRD1 (glucose-ribitol dehydrogenase 1), which shows enzymatic activity on cellobiose and positively influences cellulase gene transcription, expression, and extracellular endo-1,4-β-D-glucanase activity. grd1 is differentially transcribed upon growth on cellulose and the induction of cellulase gene expression by sophorose. The transcription of grd1 is coregulated with that of cel7a (cbh1) under inducing conditions. GRD1 is further involved in carbon source utilization on several carbon sources, such as those involved in lactose and D-galactose catabolism, in several cases in a light-dependent manner. We conclude that GRD1 represents a novel enhancer of cellulase gene expression, which by coregulation with the major cellulase may act via optimization of inducing mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellobiose / metabolism
  • Cellulase / metabolism*
  • Cluster Analysis
  • Gene Expression Profiling
  • Gene Expression Regulation, Fungal
  • Gene Order
  • Glucans / metabolism
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism*
  • Phylogeny
  • Regulon*
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transcriptional Activation
  • Trichoderma / enzymology*
  • Trichoderma / genetics

Substances

  • Glucans
  • Cellobiose
  • Oxidoreductases
  • Cellulase
  • sophorose