Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae

FEBS Lett. 1990 Apr 9;263(1):93-8. doi: 10.1016/0014-5793(90)80713-s.

Abstract

Funiculosin is a well-known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH2-cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo-cytochrome b mutants resistant to this inhibitor. Funiculosin-resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Anthraquinones / pharmacology
  • Antifungal Agents / pharmacology*
  • Cytochrome b Group / genetics*
  • Drug Resistance, Microbial / genetics
  • Electron Transport Complex III / antagonists & inhibitors*
  • Intracellular Membranes / metabolism
  • Intracellular Membranes / ultrastructure
  • Kinetics
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Molecular Sequence Data
  • Mutation*
  • NAD / metabolism
  • Oxidation-Reduction
  • Protein Conformation
  • RNA, Fungal / genetics*
  • RNA, Fungal / isolation & purification
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Sequence Homology, Nucleic Acid

Substances

  • Anthraquinones
  • Antifungal Agents
  • Cytochrome b Group
  • RNA, Fungal
  • NAD
  • funiculosin (anthraquinone)
  • Electron Transport Complex III