Isolation and characterization of microsatellite markers from the limpet Cellana grata

Mol Ecol Resour. 2009 May;9(3):902-4. doi: 10.1111/j.1755-0998.2008.02418.x. Epub 2009 Feb 11.

Abstract

This note describes the development of nine polymorphic microsatellite loci in the limpet Cellana grata to investigate population structure and cohort variation in this species. The number of alleles ranged from seven to 22 and observed heterozygosity ranged from 0.62 to 0.95. Deviation from the Hardy-Weinberg equilibrium was detected in two loci, both as a result of heterozygote deficiency. Null alleles were detected in one of these loci. These genetic markers will be used to investigate the genetic structure of C. grata populations, as well as variation among cohorts of this common intertidal species.