B cell maturation antigen deficiency exacerbates lymphoproliferation and autoimmunity in murine lupus

J Immunol. 2011 Jun 1;186(11):6136-47. doi: 10.4049/jimmunol.1001931. Epub 2011 May 2.

Abstract

Systemic lupus erythematosus and its preclinical lupus-prone mouse models are autoimmune disorders involving the production of pathogenic autoantibodies. Genetic predisposition to systemic lupus erythematosus results in B cell hyperactivity, survival of self-reactive B cells, and differentiation to autoantibody-secreting plasma cells (PCs). These corrupt B cell responses are, in part, controlled by excess levels of the cytokine BAFF that normally maintains B cell homeostasis and self-tolerance through limited production. B cell maturation Ag (BCMA) is a receptor for BAFF that, under nonautoimmune conditions, is important for sustaining enduring Ab protection by mediating survival of long-lived PCs but is not required for B cell maturation and homeostasis. Through analysis of two different lupus-prone mouse models deficient in BCMA, we identify BCMA as an important factor in regulating peripheral B cell expansion, differentiation, and survival. We demonstrate that a BCMA deficiency combined with the lpr mutation or the murine lupus susceptibility locus Nba2 causes dramatic B cell and PC lymphoproliferation, accelerated autoantibody production, and early lethality. This study unexpectedly reveals that BCMA works to control B cell homeostasis and self-tolerance in systemic autoimmunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoimmunity / immunology*
  • B-Cell Activating Factor / immunology
  • B-Cell Activating Factor / metabolism
  • B-Cell Maturation Antigen / deficiency*
  • B-Cell Maturation Antigen / genetics
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Cell Differentiation / immunology
  • Female
  • Flow Cytometry
  • Immunoblotting
  • Kaplan-Meier Estimate
  • Lupus Erythematosus, Systemic / genetics
  • Lupus Erythematosus, Systemic / immunology*
  • Lupus Erythematosus, Systemic / metabolism
  • Lymph Nodes / immunology
  • Lymph Nodes / metabolism
  • Lymph Nodes / pathology
  • Lymphocyte Activation / immunology
  • Lymphoproliferative Disorders / genetics
  • Lymphoproliferative Disorders / immunology*
  • Lymphoproliferative Disorders / metabolism
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mutation
  • Plasma Cells / immunology
  • Plasma Cells / metabolism
  • Self Tolerance / immunology
  • Spleen / immunology
  • Spleen / metabolism
  • Spleen / pathology

Substances

  • B-Cell Activating Factor
  • B-Cell Maturation Antigen
  • Tnfsf13b protein, mouse