Detection and characterization of a multicopper oxidase from Nitrosomonas europaea

Methods Enzymol. 2011:496:423-33. doi: 10.1016/B978-0-12-386489-5.00017-8.

Abstract

Blue copper oxidase (BCO) is a multicopper oxidase (MCO) found in Nitrosomonas europaea as well as in other ammonia-oxidizing organisms. In this chapter, we detail methods used to detect, isolate, and characterize BCO from N. europaea. A method for identifying and classifying MCOs commonly found in nitrifiers based on primary sequence is also described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ammonia / metabolism
  • Crystallization
  • Nitrosomonas europaea / enzymology*
  • Oxidoreductases / analysis*
  • Oxidoreductases / chemistry*
  • Oxidoreductases / classification
  • Protein Conformation

Substances

  • Ammonia
  • Oxidoreductases
  • copper oxidase