Abstract
The chronic atrioventricular block (CAVB) dog has been widely used as an in vivo proarrhythmia model. mRNA levels of K(+) and Ca(2+) channels in the isolated ventricular tissues from normal and CAVB dogs were assayed using a real-time PCR. The mRNA levels of KvLQT1 and MiRP1 were significantly less in the CAVB heart compared with those in the intact heart, whereas no significant difference was detected in the mRNA levels of other K(+)- or Ca(2+)-channel subunits. Adaptation against chronic bradycardia-related pathophysiology may have decreased the mRNA levels of cardiac K(+) channels, which may partly explain the arrhythmogenic property of this model.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Atrioventricular Block / metabolism*
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Bradycardia / metabolism
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Calcium Channels / genetics
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Calcium Channels / metabolism*
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Dogs
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Down-Regulation
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Female
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Heart Ventricles / metabolism
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KCNQ1 Potassium Channel / genetics
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KCNQ1 Potassium Channel / metabolism
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Male
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Myocardium / metabolism*
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Potassium Channels / genetics
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Potassium Channels / metabolism*
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Potassium Channels, Voltage-Gated / genetics
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Potassium Channels, Voltage-Gated / metabolism
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Protein Subunits / genetics
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Protein Subunits / metabolism*
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Calcium Channels
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KCNQ1 Potassium Channel
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Potassium Channels
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Potassium Channels, Voltage-Gated
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Protein Subunits
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RNA, Messenger