Association of a single-nucleotide polymorphism in the promoter region of the VEGF gene with the risk of renal cell carcinoma

Genet Test Mol Biomarkers. 2011 Sep;15(9):653-7. doi: 10.1089/gtmb.2011.0029. Epub 2011 Apr 14.

Abstract

Aims: Vascular endothelial growth factor (VEGF) protein plays an important role in tumor development and progression. Polymorphisms in the VEGF gene may lead to over- or underexpression of the protein and may be associated with either risk or progression of malignancy. The aim of this case-control study is to identify and quantify the correlation between VEGF polymorphisms and renal cell carcinoma (RCC).

Results: Restriction fragment length polymorphism methods were used for the analysis of VEGF polymorphisms at -2578 and +936 positions in the promoter and 3'-untranslated regions, respectively. The VEGF -2578 A-allele was associated with an increased risk of RCC (odds ratio: 1.6; 95% CI: 1.2-2.3) and A-carrier genotypes were strongly correlated (odds ratio: 2.7; 95% CI: 1.5-4.7) with higher risk. Comparison of VEGF +936 C/T polymorphism between patient and control groups revealed no association with renal carcinoma. Both VEGF -2578 C/A and VEGF +936 C/T polymorphisms showed no significant association with the histopathological parameters of RCC.

Conclusions: This study shows that VEGF -2578 A-allele and A-carrier genotypes are associated with an increased risk of RCC. In groups with higher incidence of RCC, a screening test for this polymorphism may be recommended in conjunction with other established markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Renal Cell / epidemiology
  • Carcinoma, Renal Cell / genetics*
  • Case-Control Studies
  • Female
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Humans
  • Kidney Neoplasms / epidemiology
  • Kidney Neoplasms / genetics*
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide* / physiology
  • Promoter Regions, Genetic / genetics*
  • Risk Factors
  • Vascular Endothelial Growth Factor A / genetics*
  • Young Adult

Substances

  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A