Genotoxicity of crude extracts of cyanobacteria from Taihu Lake on carp (Cyprinus carpio)

Ecotoxicology. 2011 Jul;20(5):1010-7. doi: 10.1007/s10646-011-0670-9. Epub 2011 Apr 7.

Abstract

Genotoxicity of crude cyanobacteria extracts (CBE) from blooms in Taihu Lake, China toward common carp (Cyprinus carpio) was measured. The primary extracellular product was determined by HPLC to be Microcystin-LR (MC-LR, L for leucine and R for arginine) with an average concentration of 2.4 × 10(2) μg MC g(-1) dry weight of cyanobacteria. Acute toxicity to carp, expressed as the 72-h LC(50,) was 53 mg, dw cyanobacteria L(-1). Genotoxicity, as determined by the micronucleus (MN) and comet assays were both dose- and time-depended. Deformities of cellular organelles in liver and gill were observed by use of transmission electron microscopy (TEM). The results showed that MC-LR from cyanobacteria from Taihu Lake could induce genotoxic response and tissue-level morphological changes in common carp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Toxins / toxicity*
  • Carps / physiology*
  • China
  • Cyanobacteria / pathogenicity*
  • Cyanobacteria Toxins
  • Gills / drug effects
  • Gills / pathology
  • Gills / ultrastructure
  • Harmful Algal Bloom
  • Liver / drug effects
  • Liver / pathology
  • Liver / ultrastructure
  • Marine Toxins / toxicity*
  • Microcystins / toxicity*
  • Mutagenicity Tests
  • Mutagens / toxicity*

Substances

  • Bacterial Toxins
  • Cyanobacteria Toxins
  • Marine Toxins
  • Microcystins
  • Mutagens