Molecular cloning, expression, and regulation of goose leptin receptor gene in adipocytes

Mol Cell Biochem. 2011 Jul;353(1-2):267-74. doi: 10.1007/s11010-011-0795-4. Epub 2011 Mar 29.

Abstract

A full-length cDNA encoding the goose (Anser anser) leptin receptor (LEPR) was cloned and sequenced. The goose LEPR gene encodes a 1,156-amino acid protein containing a signal peptide, a single transmembrane domain and specific motifs involving putative leptin-binding and signal transduction. The deduced goose LEPR protein shows more than 90% identity to duck and 75% identity to chicken and turkey. Quantitative real-time analysis reveals that the goose LEPR is predominantly expressed in brain. The expression of LEPR in goose adipocytes can be up-regulated by oleic acid in vitro. Moreover, the expression levels of genes, which have been demonstrated to be related to adipocyte differentiation, are down-regulated in LEPR-knockdown adipocytes, indicating LEPR's potential role in adipocyte differentiation in goose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Adipocytes / metabolism*
  • Amino Acid Sequence
  • Animals
  • Avian Proteins / genetics*
  • Brain / metabolism
  • Cell Differentiation / genetics
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Dose-Response Relationship, Drug
  • Geese / genetics*
  • Geese / metabolism
  • Gene Expression Profiling*
  • Gene Expression Regulation / drug effects
  • Molecular Sequence Data
  • Oleic Acid / pharmacology
  • RNA Interference
  • Receptors, Leptin / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Up-Regulation / drug effects
  • Up-Regulation / genetics

Substances

  • Avian Proteins
  • DNA, Complementary
  • Receptors, Leptin
  • Oleic Acid

Associated data

  • GENBANK/HQ699480