How to embed three-dimensional flexible electrodes in microfluidic devices for cell culture applications

Lab Chip. 2011 May 7;11(9):1593-5. doi: 10.1039/c1lc20084d. Epub 2011 Mar 24.

Abstract

This communication describes a simple, rapid and cost effective method of embedding a conductive and flexible material within microfluidic devices as a means to realize uniform electric fields within cellular microenvironments. Fluidic channels and electrodes are fabricated by traditional soft-lithography in conjunction with chemical etching of PDMS. Devices can be deformable (thus allowing for a combination of electro-mechanical stimulation), they are made from inexpensive materials and easily assembled by hand; this method is thus accessible to a wide range of laboratories and budgets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cell Line
  • Dimethylpolysiloxanes
  • Electrodes*
  • Electromagnetic Fields
  • Equipment Design
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Myoblasts, Cardiac
  • Nanocomposites
  • Nylons
  • Rats

Substances

  • Dimethylpolysiloxanes
  • Nylons
  • poly(dimethylsiloxane)-polyamide copolymer