A general method for affinity-based proteomic profiling of exo-α-glycosidases

Chem Commun (Camb). 2011 May 7;47(17):5037-9. doi: 10.1039/c1cc10308c. Epub 2011 Mar 22.

Abstract

Synthesis of iminosugar-based affinity-based proteomics probes for use in probing exo-α-glycosidase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Deoxynojirimycin / pharmacology
  • Affinity Labels / chemical synthesis
  • Affinity Labels / metabolism*
  • Arabidopsis
  • Cross-Linking Reagents / chemistry
  • Enzyme Inhibitors / pharmacology
  • Escherichia coli K12
  • Glycoside Hydrolases / antagonists & inhibitors
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / metabolism*
  • Photochemical Processes
  • Proteomics / methods
  • Substrate Specificity
  • Ultraviolet Rays
  • Yeasts

Substances

  • Affinity Labels
  • Cross-Linking Reagents
  • Enzyme Inhibitors
  • 1-Deoxynojirimycin
  • Glycoside Hydrolases