Abstract
Ratiometric pH nanosensors with tuneable pK(a) were prepared by entrapping combinations of two pH-sensitive fluorophores (fluorescein isothiocyanate dextran (FITC-D) and Oregon Green(®) dextran (OG-D)) and a reference fluorophore (5-(and-6)-carboxytetramethylrhodamine dextran (TAMRA-D)), in a biocompatible polymer matrix. Dual-fluorophore pH nanosensors permit the measurement of an extended dynamic range, from pH 4.0 to 7.5.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biocompatible Materials / metabolism
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Biosensing Techniques / methods*
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Dextrans / chemistry
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Fluorescein-5-isothiocyanate / analogs & derivatives
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Fluorescein-5-isothiocyanate / chemistry
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Fluorescent Dyes / chemistry*
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Hydrogen-Ion Concentration
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Ionophores*
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Nanoparticles / chemistry
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Nanotechnology
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Polymers / chemistry*
Substances
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Biocompatible Materials
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Dextrans
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Fluorescent Dyes
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Ionophores
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Polymers
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fluorescein isothiocyanate dextran
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Fluorescein-5-isothiocyanate