Cloned Drosophila DNA fragments containing telomere-specific sequences have been hybridized in situ. It is found that intensity of their hybridization with telomeres greatly varies in different nuclei of the same salivary gland. This phenomenon is also observed for internal sites where some mobile elements included in several DNA fragments under investigation are located. Within each nucleus different regions are hybridized non-uniformly as well. It is suggested that these phenomena can be explained by varying polytenization in telomeres and some internal chromosomal regions.