A specific and sensitive method for detection of hypochlorous acid for the imaging of microbe-induced HOCl production

Chem Commun (Camb). 2011 Apr 21;47(15):4373-5. doi: 10.1039/c1cc10589b. Epub 2011 Mar 14.

Abstract

A specific and sensitive fluorescence-based method was developed for the imaging of microbe-induced HOCl production. Furthermore, we demonstrate dual oxidase (DUOX)-mediated HOCl generation in the mucosa of live animals providing a novel insight into mucosal innate immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Physiological Phenomena*
  • Cell Survival
  • Drosophila melanogaster / immunology
  • Drosophila melanogaster / microbiology
  • Fungi / physiology*
  • Humans
  • Hypochlorous Acid / metabolism*
  • Immunity, Innate
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / microbiology
  • Molecular Imaging / methods*
  • NADPH Oxidases / metabolism
  • Neutrophils / cytology
  • Neutrophils / microbiology
  • Phagosomes / metabolism
  • Rhodamines / chemistry

Substances

  • Rhodamines
  • dual oxidase, Drosophila
  • Hypochlorous Acid
  • NADPH Oxidases