Abstract
Introduction:
This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil.
Methodology:
Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays.
Results:
It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1.
Conclusion:
The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.
MeSH terms
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Abattoirs*
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Animals
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Bacterial Toxins / genetics
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Brazil / epidemiology
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Carrier State / epidemiology
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Carrier State / microbiology
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Carrier State / veterinary*
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Enterotoxigenic Escherichia coli / genetics
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Enterotoxigenic Escherichia coli / isolation & purification*
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Enterotoxins / genetics
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Escherichia coli Infections / epidemiology
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Escherichia coli Proteins / genetics
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Intestines / microbiology
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Polymerase Chain Reaction / methods
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Prevalence
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Shiga Toxin 1 / genetics
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Shiga Toxin 2 / genetics
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Shiga-Toxigenic Escherichia coli / genetics
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Shiga-Toxigenic Escherichia coli / isolation & purification*
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Swine
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Swine Diseases / epidemiology*
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Swine Diseases / microbiology
Substances
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Bacterial Toxins
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Enterotoxins
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Escherichia coli Proteins
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Shiga Toxin 1
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Shiga Toxin 2
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heat-labile enterotoxin, E coli