Identification of Streptococcus pneumoniae Cps2C residues that affect capsular polysaccharide polymerization, cell wall ligation, and Cps2D phosphorylation

J Bacteriol. 2011 May;193(9):2341-6. doi: 10.1128/JB.00074-11. Epub 2011 Mar 4.

Abstract

A number of single amino acid substitutions throughout Streptococcus pneumoniae Cps2C were found to affect its function and confer either a mucoid or a small colony phenotype. These mutants exhibit significant changes in capsular polysaccharide (CPS) profile relative to that of wild-type pneumococci. The introduced mutations affect either polymerization or ligation of CPS to the cell wall and/or Cps2D phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Bacterial Capsules / metabolism
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cell Wall / metabolism*
  • Gene Expression Regulation, Bacterial / physiology*
  • Mutation
  • Polysaccharides, Bacterial / genetics
  • Polysaccharides, Bacterial / metabolism*
  • Streptococcus pneumoniae / cytology
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / metabolism*

Substances

  • Bacterial Proteins
  • Polysaccharides, Bacterial