Whole-mount in situ hybridization in amphimedon

Claire Larroux; Bryony Fahey; Maja Adamska; Gemma S Richards; Marie Gauthier; Kathryn Green; Erica Lovas; Bernard M Degnan

doi:10.1101/pdb.prot5096

Whole-mount in situ hybridization in amphimedon

CSH Protoc. 2008 Dec 1:2008:pdb.prot5096. doi: 10.1101/pdb.prot5096.

Authors

Claire Larroux¹, Bryony Fahey, Maja Adamska, Gemma S Richards, Marie Gauthier, Kathryn Green, Erica Lovas, Bernard M Degnan

Affiliation

¹ School of Integrative Biology, University of Queensland, Brisbane QLD 4072, Australia.

PMID: 21356742
DOI: 10.1101/pdb.prot5096

Abstract

INTRODUCTIONDevelopmental gene expression is analyzed predominantly via whole-mount in situ hybridization using digoxigenin-labeled RNA probes. This protocol describes how to perform this procedure in Amphimedon queenslandica, including fixation, hybridization, and sectioning of embryonic, larval, and post-larval juvenile stages.