Kaposi's sarcoma-associated herpesvirus bacterial artificial chromosome contains a duplication of a long unique-region fragment within the terminal repeat region

Yuri Yakushko; Christian Hackmann; Thomas Günther; Jessica Rückert; Marc Henke; Lars Koste; Khaled Alkharsah; Jens Bohne; Adam Grundhoff; Thomas F Schulz; Cornelia Henke-Gendo

doi:10.1128/JVI.02068-10

Kaposi's sarcoma-associated herpesvirus bacterial artificial chromosome contains a duplication of a long unique-region fragment within the terminal repeat region

J Virol. 2011 May;85(9):4612-7. doi: 10.1128/JVI.02068-10. Epub 2011 Feb 9.

Authors

Yuri Yakushko¹, Christian Hackmann, Thomas Günther, Jessica Rückert, Marc Henke, Lars Koste, Khaled Alkharsah, Jens Bohne, Adam Grundhoff, Thomas F Schulz, Cornelia Henke-Gendo

Affiliation

¹ Institute of Virology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.

Abstract

Use of the Kaposi's sarcoma-associated herpesvirus (KSHV) bacterial artificial chromosome 36 (KSHV-BAC36) genome permits reverse genetics approaches to study KSHV biology. While sequencing the complete KSHV-BAC36 genome, we noted a duplication of a 9-kb fragment of the long unique region in the terminal repeat region. This duplication covers a part of open reading frame (ORF) 19, the complete ORFs 18, 17, 16, K7, K6, and K5, and the putative ORF in the left origin of lytic replication, and it contains the BAC cassette. This observation needs to be kept in mind if viral genes located within the duplicated region are to be mutated in KSHV-BAC36.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes, Artificial, Bacterial*
DNA, Viral / chemistry
DNA, Viral / genetics
Herpesvirus 8, Human / genetics*
Molecular Sequence Data
Open Reading Frames
Segmental Duplications, Genomic*
Sequence Analysis, DNA
Terminal Repeat Sequences*

Substances

DNA, Viral

Associated data

GENBANK/HQ404500