DNA microarray analysis of human coronary artery endothelial cells exposed to cadmium

J Toxicol Sci. 2011 Jan;36(1):141-3. doi: 10.2131/jts.36.141.

Abstract

Cadmium (Cd) is a toxic heavy metal that has been shown to induce vascular diseases, such as atherosclerosis. We used DNA microarray to monitor the transcriptional response of human coronary artery endothelial cells (HCAEC) to a non-lethal dose of Cd (10 µM). Out of 35,035 human genes, Cd enhanced the expression of 3 metallothionein (MT)-I subisoform genes, including MT1E, MT1H and MT1B, and reduced the expression of 12 genes, including ISG20 and TK1, 2-fold or greater.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Atherosclerosis / etiology
  • Cadmium Chloride / toxicity*
  • Cells, Cultured
  • Down-Regulation
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism*
  • Exonucleases / genetics*
  • Exoribonucleases
  • Gene Expression / drug effects*
  • Humans
  • Metallothionein / genetics*
  • Oligonucleotide Array Sequence Analysis*
  • RNA / analysis*
  • Thymidine Kinase / genetics*
  • Up-Regulation

Substances

  • RNA
  • Metallothionein
  • Thymidine Kinase
  • thymidine kinase 1
  • Exonucleases
  • Exoribonucleases
  • ISG20 protein, human
  • Cadmium Chloride